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Anti-MAP1LC3B Antibody (3M54)
还可以产品编号 TMAH-00723
别名 Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B
Anti-MAP1LC3B Antibody (3M54) 是一种抗体，靶向 MAP1LC3B。Anti-MAP1LC3B Antibody (3M54) 可用于 ELISA, WB, FC。
Anti-MAP1LC3B Antibody (3M54)
Anti-MAP1LC3B Antibody (3M54)
还可以产品编号 TMAH-00723 别名 Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B
Anti-MAP1LC3B Antibody (3M54) 是一种抗体，靶向 MAP1LC3B。Anti-MAP1LC3B Antibody (3M54) 可用于 ELISA, WB, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-MAP1LC3B Antibody (3M54) is an antibody targeting MAP1LC3B. Anti-MAP1LC3B Antibody (3M54) can be used in ELISA, WB, FC. |
| 别名 | Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B |
| Ig Type | Rabbit IgG |
| 克隆号 | 3M54 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,RAW264.7 whole cell lysate,U251 whole cell lysate,Hela whole cell lysate -All lanes: LC3B antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 15 kDa -Observed band size: 15 kDa 2. Overlay Peak curve showing Hela cells stained with TMAH-00723 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, FC |
| 推荐剂量 | WB:1:500-1:2000; FC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human MAP1LC3B |
| 抗原种属 | Human |
| 基因ID | 81631 |
| Uniprot ID | |
| 研究领域 | Neuroscience, Cancer, Cardiovascular, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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