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Anti-Mafa Polyclonal Antibody
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产品编号 TMAB-01095
Anti-Mafa Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Mafa。Anti-Mafa Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Mafa Polyclonal Antibody
Anti-Mafa Polyclonal Antibody
还可以产品编号 TMAB-01095
Anti-Mafa Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Mafa。Anti-Mafa Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Mafa Polyclonal Antibody is a Rabbit antibody targeting Mafa. Anti-Mafa Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Rat (predicted:Human,Mouse) |
| 验证活性 | 1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Mafa Polyclonal Antibody, Unconjugated (TMAB-01095) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Rat Pancreas tissue lysates Primary: Anti-Mafa (TMAB-01095) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 47 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. Note=Detected in nuclei of pancreas islet beta cells. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development. |
| 免疫原 | KLH conjugated synthetic peptide: mouse Mafa |
| 抗原种属 | Mouse |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Co-factors,Other factors,Insulin / Insulin-like |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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