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Anti-LOX-1 Polyclonal Antibody

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Anti-LOX-1 Polyclonal Antibody
产品编号 TMAB-01080

Anti-LOX-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 LOX-1。Anti-LOX-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-LOX-1 Polyclonal Antibody

Anti-LOX-1 Polyclonal Antibody

Rating icon 还可以
Anti-LOX-1 Polyclonal Antibody
产品编号 TMAB-01080

Anti-LOX-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 LOX-1。Anti-LOX-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-LOX-1 Polyclonal Antibody is a Rabbit antibody targeting LOX-1. Anti-LOX-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat,Rabbit (predicted:Cow)
验证活性
1. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-LOX 1 (TMAB-01080) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kDa
Observed band size: 50 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-LOX1 (TMAB-01080) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kDa
Observed band size: 50 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Single-pass type II membrane protein. Secreted. Note=A secreted form also exists.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
抗原信息
免疫原
KLH conjugated synthetic peptide: rabbit LOX-1
抗原种属
Rabbit
研究领域
Cholesterol Metabolism,Cholesterol Metabolism
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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