Anti-LOX-1 Polyclonal Antibody is a Rabbit antibody targeting LOX-1. Anti-LOX-1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat,Rabbit (predicted:Cow)

1. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-LOX 1 (TMAB-01080) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kDa
Observed band size: 50 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-LOX1 (TMAB-01080) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: rabbit LOX-1

Rabbit

OLR1

Oxidized low-density lipoprotein receptor 1

Cholesterol Metabolism,Cholesterol Metabolism

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.