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Anti-LOX-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 LOX-1。Anti-LOX-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-LOX-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 LOX-1。Anti-LOX-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-LOX-1 Polyclonal Antibody is a Rabbit antibody targeting LOX-1. Anti-LOX-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,Rabbit (predicted:Cow) |
验证活性 | 1. Sample: 293t (Human) Cell Lysate at 30 μg Primary: Anti-LOX 1 (TMAB-01080) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (TMAB-01080) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Sample: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-LOX1 (TMAB-01080) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kDa Observed band size: 50 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell membrane; Single-pass type II membrane protein. Secreted. Note=A secreted form also exists. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. |
免疫原 | KLH conjugated synthetic peptide: rabbit LOX-1 |
抗原种属 | Rabbit |
研究领域 | Cholesterol Metabolism,Cholesterol Metabolism |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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