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Anti-LIPT2 Polyclonal Antibody

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货号 TMAB-08327

Anti-LIPT2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 LIPT2。Anti-LIPT2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-LIPT2 Polyclonal Antibody

Anti-LIPT2 Polyclonal Antibody

一键复制产品信息
Rating icon 还可以
货号 TMAB-08327

Anti-LIPT2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 LIPT2。Anti-LIPT2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,785
5日内发货
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颜色: 无色透明
性状: Liquid
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产品介绍

生物活性
产品描述
Anti-LIPT2 Polyclonal Antibody is a Rabbit antibody targeting LIPT2. Anti-LIPT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse (predicted:Rat,Dog,Horse)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LIPT2) Polyclonal Antibody, Unconjugated (TMAB-08327) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: A431 (Human) Cell Lysate at 40 μg
Primary: Anti-LIPT2 (TMAB-08327) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Mitochondrion.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景LIPT2 is a 231 amino acid mitochondrial protein that belongs to the LipB family. LIPT2 catalyzes the exchange of octanoic acid from octanoyl-acyl-carrier-protein to lipoate-dependent enzymes. The gene encoding LIPT2 maps to human chromosome 11, which comprises approximately 4% of human genomic DNA and is considered a gene and disease association dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and thalassemia are caused by HBB gene mutations, while Wilms' tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are also associated with defects in chromosome 11-encoded genes.
抗原信息
免疫原
KLH conjugated synthetic peptide: human LIPT2
抗原种属
Human
基因名称
LIPT2
基因ID
387787
蛋白名称
Putative lipoyltransferase 2, mitochondrial
Uniprot ID
A6NK58
功能
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
化学信息
分子量Theoretical: 23 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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