Anti-Leptin Receptor Antibody (1S966) is a Rabbit antibody targeting Leptin Receptor. Anti-Leptin Receptor Antibody (1S966) can be used in IHC-P,IHC-Fr,IF,WB.

IgG

1S966

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
12. 25 ug total protein per lane of various lysates (see on figure) probed with Leptin Receptor monoclonal antibody, unconjugated (TMAB-08281) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
13. Paraformaldehyde-fixed, paraffin embedded human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.
14. Paraformaldehyde-fixed, paraffin embedded human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Leptin Receptor Monoclonal Antibody, Unconjugated (TMAB-08281) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; WB: 1:500-2000

Monoclonal

A synthesized peptide: human Leptin receptor

Human

LEPR

Leptin receptor

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).