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Anti-LC3B Antibody (7R171)

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货号 TMAB-08239

Anti-LC3B Antibody (7R171) 是一种 Rabbit 抗体，靶向 LC3B。Anti-LC3B Antibody (7R171) 可用于 IF, IHC-Fr, IHC-P, WB。

Anti-LC3B Antibody (7R171)

Anti-LC3B Antibody (7R171)

一键复制产品信息
Rating icon 还可以
货号 TMAB-08239

Anti-LC3B Antibody (7R171) 是一种 Rabbit 抗体，靶向 LC3B。Anti-LC3B Antibody (7R171) 可用于 IF, IHC-Fr, IHC-P, WB。

规格价格库存数量
25 μL
¥ 795
5日内发货
50 μL
¥ 1,380
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-LC3B Antibody (7R171) is a Rabbit antibody targeting LC3B. Anti-LC3B Antibody (7R171) can be used in IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG/Kappa
克隆号
7R171
反应种属
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human right occipital lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. 25 ug total protein per lane of various lysates (see on figure) probed with LC3B monoclonal antibody, unconjugated (TMAB-08239) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用IFIHC-FrIHC-PWB
推荐剂量
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:1000-2000
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes.
组织特异性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
抗原信息
免疫原
A synthesized peptide: human LC3B
抗原种属
Human
基因名称
MAP1LC3B
基因ID
81631
蛋白名称
Microtubule-associated proteins 1A/1B light chain 3B
Uniprot ID
Q9GZQ8
功能
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
化学信息
分子量Theoretical: 15 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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