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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-LC3A/B Polyclonal Antibody

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货号 TMAB-08238

Anti-LC3A/B Polyclonal Antibody 是一种 Rabbit 抗体，靶向 LC3A/B。Anti-LC3A/B Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。

Anti-LC3A/B Polyclonal Antibody

Anti-LC3A/B Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-08238

Anti-LC3A/B Polyclonal Antibody 是一种 Rabbit 抗体，靶向 LC3A/B。Anti-LC3A/B Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-LC3A/B Polyclonal Antibody is a Rabbit antibody targeting LC3A/B. Anti-LC3A/B Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
反应种属
Human,Mouse (predicted:Rat,Cow,Horse,Sheep,GuineaPig,Goat)
验证活性
1. Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-LC3A/B antibody (TMAB-08238), Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LC3A/B) Polyclonal Antibody, Unconjugated (TMAB-08238) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用IHC-PIHC-FrIFFCM
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes.
组织特异性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
浓度1mg/ml
研究背景A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
抗原信息
免疫原
KLH conjugated synthetic peptide: human LC3A/B
抗原种属
Human
基因名称
MAP1LC3B
基因ID
81631
蛋白名称
Microtubule-associated proteins 1A/1B light chain 3B
Uniprot ID
Q9GZQ8
功能
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
化学信息
分子量Theoretical: 14 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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