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顶部生物活性储存和溶解信息计算器

Anti-Latexin Antibody (7N890)

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Anti-Latexin Antibody (7N890)
产品编号 TMAY-00472
别名 latexin

Anti-Latexin Antibody (7N890) 是一种 Mouse 抗体，靶向 Latexin。Anti-Latexin Antibody (7N890) 可用于 WB,ELISA,IP。

Anti-Latexin Antibody (7N890)
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Anti-Latexin Antibody (7N890)

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Anti-Latexin Antibody (7N890)
产品编号 TMAY-00472 别名 latexin

Anti-Latexin Antibody (7N890) 是一种 Mouse 抗体，靶向 Latexin。Anti-Latexin Antibody (7N890) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 1,679
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产品介绍

生物活性
产品描述
Anti-Latexin Antibody (7N890) is a Mouse antibody targeting Latexin. Anti-Latexin Antibody (7N890) can be used in WB,ELISA,IP.
别名latexin
Ig Type
Mouse IgG1
克隆号
7N890
交叉反应
Human
验证活性
1. Anti-LXN mouse monoclonal antibody at 1:500 dilution.
-Lane A: A549 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:26 kDa.
-Observed band size:26 kDa.
2. LXN was immunoprecipitated using:
-Lane A:0.5 mg MCF-7 Whole Cell Lysate
-0.5 µL anti-LXN mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-LXN mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 25.7 kDa.
-Observed band size: 27 kDa
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 1-2 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Latexin (rh Latexin; TMPY-00870; NP_064554.3; Glu 2-Glu 222). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Latexin protein (TMPY-00870)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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