Anti-Latent TGF-beta 2 Polyclonal Antibody is a Rabbit antibody targeting Latent TGF-beta 2. Anti-Latent TGF-beta 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Rabbit,Cow)

1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated (TMAB-01049) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Kidney (Mouse) lysate at 40 μg
Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12/45 kDa
Observed band size: 50 kDa
3. Sample: MCF-7 (Human) cell Lysate at 40 μg
Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12/45 kDa
Observed band size: 48 kDa
4. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-TGF beta 2 Propeptide (TMAB-01049) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/45 kDa
Observed band size: 48 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TGF-beta 2

Human

Response to hypoxia,TGF,Angiogenic growth factors,Hypoxia,TGF,Secreted