Anti-Laminin subunit beta-1 Polyclonal Antibody is a Rabbit antibody targeting Laminin subunit beta-1. Anti-Laminin subunit beta-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat (predicted:Cow)

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
8. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Skin (Mouse) Lysate at 40 μg
Lane 3: Placenta (Mouse) Lysate at 40 μg
Lane 4: U2os (Human) Cell Lysate at 30 μg
Lane 5: Huvec (Human) Cell Lysate at 30 μg
Lane 6: HepG2 (Human) Cell Lysate at 30 μg
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 8: A549 (Human) Cell Lysate at 30 μg
Lane 9: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-laminin (TMAB-01044) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 220-240 kDa
Observed band size: 240 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human Laminin subunit beta-1

Human