Anti-Lamin A/C Antibody (5X372) is a Rabbit antibody targeting Lamin A/C. Anti-Lamin A/C Antibody (5X372) can be used in WB,ICC/IF,FCM.

IgG

5X372

Human,Mouse,Rat

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Lamin A/C) monoclonal Antibody, unconjugated (TMAB-08196) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Lamin A/C antibody (TMAB-08196, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 μg total protein per lane of various lysates (see on figure) probed with Lamin A/C monoclonal antibody, unconjugated (TMAB-08196) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Lamin A/C

Human

LMNA

Prelamin-A/C

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics.