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Anti-KRT5 Antibody (6A459)
还可以产品编号 TMAY-00123
Anti-KRT5 Antibody (6A459) 是一种 Rabbit 抗体，靶向 KRT5。Anti-KRT5 Antibody (6A459) 可用于 WB,ICC/IF。
Anti-KRT5 Antibody (6A459)
Anti-KRT5 Antibody (6A459)
还可以产品编号 TMAY-00123
Anti-KRT5 Antibody (6A459) 是一种 Rabbit 抗体，靶向 KRT5。Anti-KRT5 Antibody (6A459) 可用于 WB,ICC/IF。
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抗原信息
| 产品描述 | Anti-KRT5 Antibody (6A459) is a Rabbit antibody targeting KRT5. Anti-KRT5 Antibody (6A459) can be used in WB,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6A459 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-KRT5 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:62 kDa. -Observed band size:62 kDa(We are unsure as to the identity of these extra bands.) 2. Immunofluorescence staining of KRT5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KRT5 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm . |
| 应用 | WB,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5/KRT5. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Keratin 5 is the major type II keratin of the basal cells of the epidermis and other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the center region of the Human Cytokeratin 5/KRT5 |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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