Anti-KMT4 Polyclonal Antibody is a Rabbit antibody targeting KMT4. Anti-KMT4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Cow,Sheep,African Green Monkey)

1. Sample:
Liver (Mouse) Lysate at 40 μg
Liver (Rat) Lysate at 40 μg
Primary: Anti-KMT4 (TMAB-08141) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 185 kD
Observed band size: 170 kD
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated (TMAB-08141) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human KMT4

Human

DOT1L

Histone-lysine N-methyltransferase, H3 lysine-79 specific

The KMT4 / Dot1 (disruptor of telomeric silencing) family are a class of histone methyltransferases that play an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications.