Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM.

IgG

5I460

Human,Mouse,Rat

1. Western blot analysis of SH-SY5Y cell lysate. Using KIF3A (TMAB-08047) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-KIF3A antibody (TMAB-08047, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human KIF3A

Human

KIF3A

Kinesin-like protein KIF3A

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.