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Anti-KIBRA Polyclonal Antibody
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产品编号 TMAB-01021
Anti-KIBRA Polyclonal Antibody 是一种 Rabbit 抗体，靶向 KIBRA。Anti-KIBRA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-KIBRA Polyclonal Antibody
Anti-KIBRA Polyclonal Antibody
还可以产品编号 TMAB-01021
Anti-KIBRA Polyclonal Antibody 是一种 Rabbit 抗体，靶向 KIBRA。Anti-KIBRA Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-KIBRA Polyclonal Antibody is a Rabbit antibody targeting KIBRA. Anti-KIBRA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Cow,Rabbit,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Siha (Human) Cell Lysate at 30 μg A431 (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg U-2Os (Human) Cell Lysate at 30 μg Primary: Anti-KIBRA (TMAB-01021) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kDa Observed band size:125 kDa 6. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (TMAB-01021) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell projection, ruffle membrane. Note=Co-localizes with PRKCZ in the perinuclear region. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | KIBRA is a 1,113 amino acid protein that localizes to the cytoplasm and contains one C2 domain and two WW domains. Expressed in colon, brain, kidney and heart tissue, KIBRA is thought to interact with dendrin and, via this interaction, may play a role in collagen-induced signaling. Additionally, KIBRA, which exists as multiple alternatively spliced isoforms, is involved in memory performance and in the pathogenesis of Alzheimer's disease. The gene encoding KIBRA maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| 免疫原 | KLH conjugated synthetic peptide: human KIBRA |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Neural Signal Transduction,Cytoplasmic |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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