Anti-ITGB3 Polyclonal Antibody is a Rabbit antibody targeting ITGB3. Anti-ITGB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Pig,Cow,Rabbit)

1. Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (TMAB-00997) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Bone Lysates
Lane 2: Mouse Kidney Lysates
Primary: Anti-Integrin beta 3 (TMAB-00997) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Integrin beta 3

Human

Centromere,Centromeres,NFkB pathway,Co-activators/co-repressors,Beta,NFkB Pathway,Co-activators/co-repressors