Anti-IRS-2 Polyclonal Antibody 2 is a Rabbit antibody targeting IRS-2. Anti-IRS-2 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IRS2 Polyclonal Antibody, Unconjugated (TMAB-07812) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRS-2) Polyclonal Antibody, Unconjugated (TMAB-07812) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (Red line): Rabbit Anti-IRS-2 antibody (TMAB-07812);
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (green line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1 μg /test.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human IRS-2

Human

IRS2

Insulin receptor substrate 2

May mediate the control of various cellular processes by insulin.