Anti-iNOS Polyclonal Antibody is a Rabbit antibody targeting iNOS. Anti-iNOS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

IgG

Human,Mouse,Rat

1. Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (TMAB-00970) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (TMAB-00970) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: U251 Cell Lysate at 40 μg
Primary: Anti-iNOs (TMAB-00970) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 130 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (TMAB-00970) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (TMAB-00970) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
Lane 1: Mouse Raw264.7 tissue lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-iNOS (TMAB-00970) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 140 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human NOS-2

Human

Cellular metabolic process,Response to hypoxia,Hypoxia,NOS