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Anti-INHBB Polyclonal Antibody

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Anti-INHBB Polyclonal Antibody
产品编号 TMAB-00969

Anti-INHBB Polyclonal Antibody 是一种 Rabbit 抗体,靶向 INHBB。Anti-INHBB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-INHBB Polyclonal Antibody

Anti-INHBB Polyclonal Antibody

Rating icon 还可以
Anti-INHBB Polyclonal Antibody
产品编号 TMAB-00969

Anti-INHBB Polyclonal Antibody 是一种 Rabbit 抗体,靶向 INHBB。Anti-INHBB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-INHBB Polyclonal Antibody is a Rabbit antibody targeting INHBB. Anti-INHBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep,GuineaPig)
验证活性
1. Sample:
A431 (Human) Cell Lysate at 40 μg
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
2. Sample:
Brain (Mouse) Lysate at 40 μg
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (TMAB-00969) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Mouse Liver tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Breast tissue lysates
Lane 7: Human A431 cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/45 kDa
Observed band size: 46 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq].
抗原信息
免疫原
KLH conjugated synthetic peptide: human Inhibin beta B
抗原种属
Human
基因ID
Uniprot ID
研究领域
Gonadotrophic axis,Hypothalamic pituitary adrenal axis,Hormones
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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