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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-ILVBL Polyclonal Antibody

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货号 TMAB-07686

Anti-ILVBL Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ILVBL。Anti-ILVBL Polyclonal Antibody 可用于 WB,FCM。

Anti-ILVBL Polyclonal Antibody

Anti-ILVBL Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-07686

Anti-ILVBL Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ILVBL。Anti-ILVBL Polyclonal Antibody 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-ILVBL Polyclonal Antibody is a Rabbit antibody targeting ILVBL. Anti-ILVBL Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat,Dog,Pig,Horse)
验证活性
1. Sample:
Hcclm3 (Human) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-ILVBL (TMAB-07686) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
2. Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-ILVBL antibody (TMAB-07686)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample: HepG2 Cell (Human) Lysate at 30 μg
Primary: Anti-ILVBL (TMAB-07686) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 3μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane.
组织特异性Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景ILVBL is a 632 amino acid single-pass membrane protein that belongs to the TPP enzyme family. Expressed in the majority of tissues, ILVBL has the highest level of expression in heart, pancreas and placenta. ILVBL is highly homologous to several bacterial enzymes, including the B isozyme of the large catalytic subunit of E. coli acetohydroxy-acid synthase (AHAS) and the oxalyl-coA decarboxylase of O. formigenes, that utilize thiamine pyrophosphate as a cofactor. ILVBL binds one magnesium ion and one thiamine pyrophosphate per subunit, and may catalyze the initial step in branched-chain amino acid biosynthesis. The gene encoding ILVBL maps to human chromosome 19p13.12 and mouse chromosome 10 C1.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ILVBL
抗原种属
Human
基因名称
ILVBL
基因ID
10994
蛋白名称
2-hydroxyacyl-CoA lyase 2
Uniprot ID
A1L0T0
化学信息
分子量Theoretical: 68 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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