Anti-IL12B Polyclonal Antibody 2 is a Rabbit antibody targeting IL12B. Anti-IL12B Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Sheep)

1. Protein: spleen (mouse) lysates at 40 μg;
Primary: Anti-Il-12 beta (TMAB-07599) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
ECL excitated the fluorescence;
Predicted band size: 35 kD
Observed band size: 37 kD
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IL-12 beta Polyclonal Antibody, Unconjugated (TMAB-07599) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IL12B

Human

IL-12B

Interleukin-12 subunit beta