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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-IL-5 Polyclonal Antibody

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货号 TMAB-00963

别名 白细胞介素, TRF, interleukin 5, IL-5, EDF

Anti-IL-5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-5。Anti-IL-5 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-IL-5 Polyclonal Antibody

Anti-IL-5 Polyclonal Antibody

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货号 TMAB-00963 别名 白细胞介素, TRF, interleukin 5, IL-5, EDF

Anti-IL-5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-5。Anti-IL-5 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-IL-5 Polyclonal Antibody is a Rabbit antibody targeting IL-5. Anti-IL-5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名白细胞介素, TRF, interleukin 5, IL-5, EDF
Ig Type
IgG
反应种属
Human,Rat (predicted:Mouse)
验证活性
1. Sample:
LOVO Cell (Human) Lysate at 30 μg
Primary: Anti-IL-5 (TMAB-00963) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 10 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (TMAB-00963) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (TMAB-00963) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (TMAB-00963) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Interleukin 5 is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology.
抗原信息
免疫原
KLH conjugated synthetic peptide: mouse IL-5
抗原种属
Mouse
基因名称
IL5
蛋白名称
Interleukin-5
研究领域
Interleukins,Non-CD,Interleukins
功能
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
化学信息
分子量Theoretical: 13 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

  • 稀释 计算器

关键词

IL5IL 5
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