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顶部生物活性储存和溶解信息计算器

Anti-IL-3 Antibody (8Y609)

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产品编号 TMAY-03784

别名 白介素, PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA

Anti-IL-3 Antibody (8Y609) 是一种 Mouse 抗体，靶向 IL-3。Anti-IL-3 Antibody (8Y609) 可用于 IP,WB。

Anti-IL-3 Antibody (8Y609)

Anti-IL-3 Antibody (8Y609)

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Rating icon 还可以
产品编号 TMAY-03784 别名 白介素, PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA

Anti-IL-3 Antibody (8Y609) 是一种 Mouse 抗体，靶向 IL-3。Anti-IL-3 Antibody (8Y609) 可用于 IP,WB。

规格价格库存数量
100 μL
¥ 1,684
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产品介绍

生物活性
产品描述
Anti-IL-3 Antibody (8Y609) is a Mouse antibody targeting IL-3. Anti-IL-3 Antibody (8Y609) can be used in IP,WB.
别名白介素, PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA
Ig Type
Mouse IgG1
克隆号
8Y609
交叉反应
Rat
特异性
Rat IL-3
验证活性
1. Anti-IL3 mouse monoclonal antibody at 1:500 dilution.
-Lane A: Rat heart tissue lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:17 kDa.
-Observed band size:17 kDa.
2. IL3 was immunoprecipitated using:
-Lane A:0.5 mg A431 Whole Cell Lysate.
-4 µL anti-IL3 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-IL3 mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 17 kDa.
-Observed band size:18 kDa
应用IPWB
推荐剂量
WB: 1:500-1:2000; IP: 5-10 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat IL3 (rR IL3; TMPY-02772; NP_113701.1; Met1-Cys169). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Rat IL3 Protein (TMPY-02772)
抗原种属
Rat
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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