Anti-IL-11 Polyclonal Antibody is a Rabbit antibody targeting IL-11. Anti-IL-11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,GuineaPig)

1. Sample: Testis (Mouse) Lysate at 30 μg
Primary: Anti-IL-11 (TMAB-00940) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (TMAB-00940) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (TMAB-00940) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
4. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-IL-11 (TMAB-00940) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IL-11

Human

Interleukins,Interleukin,Non-CD,Interleukins,Other secreted molecules,Thrombocytic Lineage