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Anti-IL-1 beta Polyclonal Antibody
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产品编号 TMAB-00932
Anti-IL-1 beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-1 beta。Anti-IL-1 beta Polyclonal Antibody 可用于 WB。
Anti-IL-1 beta Polyclonal Antibody
Anti-IL-1 beta Polyclonal Antibody
还可以产品编号 TMAB-00932
Anti-IL-1 beta Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IL-1 beta。Anti-IL-1 beta Polyclonal Antibody 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-IL-1 beta Polyclonal Antibody is a Rabbit antibody targeting IL-1 beta. Anti-IL-1 beta Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Human |
| 验证活性 | 1. Sample: Lane 1: Normal Mouse Raw264.7 cell lysates Lane 2: Raw264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours Primary: Anti-IL-1 Beta (TMAB-00932) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/32 kDa Observed band size: 35 kDa 2. THP-1 (H) cells were treated with or without TPA (80nM) for 24 h,then treated with (100ng/ml) lipopolysaccharide (LPS) for 6 h, then with (300ng/ml) Brefeldin A added after 3 h, 25 μg total protein per Lane of cell lysates probed with IL-1 Beta polyclonal antibody, unconjugated (TMAB-00932) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. |
| 免疫原 | KLH conjugated synthetic peptide: human IL-1beta |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Inflammatory mediators,Interleukins,Obesity,SARS Coronavirus |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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