Anti-IGFBP-5 Polyclonal Antibody is a Rabbit antibody targeting IGFBP-5. Anti-IGFBP-5 Polyclonal Antibody can be used in WB.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-IGFBP5 (TMAB-00924) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
2. Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated (TMAB-00924) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human IGFBP5

Human

IGFBP5

Insulin-like growth factor-binding protein 5

Insulin / Insulin-like,Lipid Signaling

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.