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Anti-IGFBP-5 Polyclonal Antibody
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产品编号 TMAB-00924
Anti-IGFBP-5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IGFBP-5。Anti-IGFBP-5 Polyclonal Antibody 可用于 WB。
Anti-IGFBP-5 Polyclonal Antibody
Anti-IGFBP-5 Polyclonal Antibody
还可以产品编号 TMAB-00924
Anti-IGFBP-5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 IGFBP-5。Anti-IGFBP-5 Polyclonal Antibody 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-IGFBP-5 Polyclonal Antibody is a Rabbit antibody targeting IGFBP-5. Anti-IGFBP-5 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Sheep) |
| 验证活性 | 1. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-IGFBP5 (TMAB-00924) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa 2. Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated (TMAB-00924) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Function : IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| 免疫原 | KLH conjugated synthetic peptide: human IGFBP5 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Insulin / Insulin-like,Lipid Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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