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Anti-ID1 Antibody (7Y466) 是一种抗体,靶向 ID1。Anti-ID1 Antibody (7Y466) 可用于 ELISA, WB, IF。
Anti-ID1 Antibody (7Y466) 是一种抗体,靶向 ID1。Anti-ID1 Antibody (7Y466) 可用于 ELISA, WB, IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,195 | 5日内发货 |
产品描述 | Anti-ID1 Antibody (7Y466) is an antibody targeting ID1. Anti-ID1 Antibody (7Y466) can be used in ELISA, WB, IF. |
别名 | bHLHb24 |
Ig Type | Rabbit IgG |
克隆号 | 7Y466 |
交叉反应 | Human, Mouse |
验证活性 | 1. Western Blot -Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate -All lanes: Id1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 17, 16 kDa -Observed band size: 26 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00590 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
应用 | ELISA, WB, IF |
推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Cytoplasm. Nucleus. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. ... |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human Id1 |
抗原种属 | Human |
基因ID | 3397 |
Uniprot ID | |
研究领域 | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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