Anti-ICAM2 Antibody (8P531) is a Rabbit antibody targeting ICAM2. Anti-ICAM2 Antibody (8P531) can be used in FCM, IF, IHC-Fr, IHC-P, IP, WB.

IgG

8P531

Mouse,Human

1. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM2 Monoclonal Antibody, Unconjugated (TMAB-07476) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM2 Monoclonal Antibody, Unconjugated (TMAB-07476) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM2 Monoclonal Antibody, Unconjugated (TMAB-07476) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with ICAM2 monoclonal antibody, unconjugated (TMAB-07476) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. The HUVEC (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ICAM2 antibody (TMAB-07476, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: mouse ICAM 2

Mouse

ICAM2

Intercellular adhesion molecule 2

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.