Anti-ICAM1 Antibody (1H180) is a Rabbit antibody targeting ICAM1. Anti-ICAM1 Antibody (1H180) can be used in WB,IF,IHC-P,IHC-Fr.

IgG

1H180

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Monoclonal Antibody, Unconjugated (TMAB-07472) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. 25 μg total protein per lane of various lysates (see on figure) probed with ICAM1 monoclonal antibody, unconjugated (TMAB-07472) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
9. 25 μg total protein per lane of various lysates (see on figure) probed with ICAM1 monoclonal antibody, unconjugated (TMAB-07472) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-5000; IF: 1:200-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000

Monoclonal

A synthesized peptide: human ICAM 1

Human

ICAM1

Intercellular adhesion molecule 1

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.