Anti-Hyaluronidase-1 Polyclonal Antibody is a Rabbit antibody targeting Hyaluronidase-1. Anti-Hyaluronidase-1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated (TMAB-07461) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated (TMAB-07461) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase-1 antibody (TMAB-07461)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 2 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: rat Haase

Rat

HYAL1

Hyaluronidase-1

May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.