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Anti-Human Lambda Light Chain Antibody (8H783)

货号 TMAB-07448 一键复制产品信息
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Anti-Human Lambda Light Chain Antibody (8H783) 是一种 Mouse 抗体,靶向 Human Lambda Light Chain。Anti-Human Lambda Light Chain Antibody (8H783) 可用于 IHC-P,WB,IHC-Fr,IF。

Anti-Human Lambda Light Chain Antibody (8H783)

一键复制产品信息
Rating icon 还可以
货号 TMAB-07448

Anti-Human Lambda Light Chain Antibody (8H783) 是一种 Mouse 抗体,靶向 Human Lambda Light Chain。Anti-Human Lambda Light Chain Antibody (8H783) 可用于 IHC-P,WB,IHC-Fr,IF。

Anti-Human Lambda Light Chain Antibody (8H783)
规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍


生物活性
产品描述
Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF.
Ig Type
IgG1
克隆号
8H783
反应种属
Human
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (TMAB-07448) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用IHC-PWBIHC-FrIF
推荐剂量
IHC-P: 1:200-1000; WB: 1:500-2000; IHC-Fr: 1:200-1000; IF: 1:200-1000
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cytoplasmic
构建方式Hybridoma Monoclonal Antibody
性状Liquid
缓冲液0.01M PBS (pH7.4).
研究背景All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
抗原信息
免疫原
Purified native protein
抗原种属
Human
基因名称
IGLC1
基因ID
蛋白名称
Immunoglobulin lambda constant 1
Uniprot ID
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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