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Anti-Human Lambda Light Chain Antibody (8H783) 是一种 Mouse 抗体,靶向 Human Lambda Light Chain。Anti-Human Lambda Light Chain Antibody (8H783) 可用于 IHC-P,WB,IHC-Fr,IF。
Anti-Human Lambda Light Chain Antibody (8H783) 是一种 Mouse 抗体,靶向 Human Lambda Light Chain。Anti-Human Lambda Light Chain Antibody (8H783) 可用于 IHC-P,WB,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
| 产品描述 | Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF. |
| Ig Type | IgG1 |
| 克隆号 | 8H783 |
| 反应种属 | Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (TMAB-07448) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | IHC-PWBIHC-FrIF |
| 推荐剂量 | IHC-P: 1:200-1000; WB: 1:500-2000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasmic |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 性状 | Liquid |
| 缓冲液 | 0.01M PBS (pH7.4). |
| 研究背景 | All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. |
| 免疫原 | Purified native protein |
| 抗原种属 | Human |
| 基因名称 | IGLC1 |
| 基因ID | |
| 蛋白名称 | Immunoglobulin lambda constant 1 |
| Uniprot ID |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |