购物车
- 全部删除
您的购物车当前为空
Anti-HSPD1 Antibody (8M190)
还可以产品编号 TMAH-00582
别名 P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin
Anti-HSPD1 Antibody (8M190) 是一种抗体，靶向 HSPD1。Anti-HSPD1 Antibody (8M190) 可用于 ELISA, WB, IHC, IP。
Anti-HSPD1 Antibody (8M190)
Anti-HSPD1 Antibody (8M190)
还可以产品编号 TMAH-00582 别名 P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin
Anti-HSPD1 Antibody (8M190) 是一种抗体，靶向 HSPD1。Anti-HSPD1 Antibody (8M190) 可用于 ELISA, WB, IHC, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-HSPD1 Antibody (8M190) is an antibody targeting HSPD1. Anti-HSPD1 Antibody (8M190) can be used in ELISA, WB, IHC, IP. |
| 别名 | P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin |
| Ig Type | Rabbit IgG |
| 克隆号 | 8M190 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue -All lanes: HSPD1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62, 18 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00582 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00582 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunoprecipitating HSPD1 in HepG2 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00582 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00582(3µg)+ HepG2 whole cell lysate(500µg) -Lane 3: HepG2 whole cell lysate (10µg) |
| 应用 | ELISA, WB, IHC, IP |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Mitochondrion matrix. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Hsp60 |
| 抗原种属 | Human |
| 基因ID | 3329 |
| Uniprot ID | |
| 研究领域 | Isotype/Loading Controls, Tags & Cell Markers, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Anti-HSPD1 Antibody (8M190) | purchase Anti-HSPD1 Antibody (8M190) | Anti-HSPD1 Antibody (8M190) cost | order Anti-HSPD1 Antibody (8M190)
嗨！有任何问题？点我咨询
评论内容