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Anti-HSPA5 Polyclonal Antibody

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货号 TMAB-00898

别名 Immunoglobulin heavy chain-binding protein, HSPA 5, HSP70 family protein 5, Heat shock protein family A member 5, Heat shock protein 70 family protein 5, GRP-78, GRP78, Endoplasmic reticulum chaperone BiP, EC 3.6.4.10, BiP, Binding-immunoglobulin protein, 78 kDa glucose-regulated protein

Anti-HSPA5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HSPA5。Anti-HSPA5 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-HSPA5 Polyclonal Antibody

Anti-HSPA5 Polyclonal Antibody

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货号 TMAB-00898 别名 Immunoglobulin heavy chain-binding protein, HSPA 5, HSP70 family protein 5, Heat shock protein family A member 5, Heat shock protein 70 family protein 5, GRP-78, GRP78, Endoplasmic reticulum chaperone BiP, EC 3.6.4.10, BiP, Binding-immunoglobulin protein, 78 kDa glucose-regulated protein

Anti-HSPA5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HSPA5。Anti-HSPA5 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品描述
Anti-HSPA5 Polyclonal Antibody is a Rabbit antibody targeting HSPA5. Anti-HSPA5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Immunoglobulin heavy chain-binding protein, HSPA 5, HSP70 family protein 5, Heat shock protein family A member 5, Heat shock protein 70 family protein 5, GRP-78, GRP78, Endoplasmic reticulum chaperone BiP, EC 3.6.4.10, BiP, Binding-immunoglobulin protein, 78 kDa glucose-regulated protein
Ig Type
IgG
反应种属
Human,Rat (predicted:Mouse,Pig)
验证活性
1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated (TMAB-00898) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Spleen (Rat) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Lane 3: U-87MG (Human) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Lane 5: HepG2 (Human) Cell Lysate at 30 μg
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-GRP78 (TMAB-00898) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (TMAB-00898) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
抗原信息
免疫原
KLH conjugated synthetic peptide: human GRP78
抗原种属
Human
基因名称
HSPA5
基因ID
3309
蛋白名称
Endoplasmic reticulum chaperone BiP
Uniprot ID
P11021
研究领域
ER
功能
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
化学信息
分子量Theoretical: 78 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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