Anti-HSP90AA1 Polyclonal Antibody is a Rabbit antibody targeting HSP90AA1. Anti-HSP90AA1 Polyclonal Antibody can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
293t (Human) Cell Lysate at 30 μg
293T KO HSP90 alpha (Human) Cell Lysate at 30 μg
Primary: Anti-HSP90 alpha (TMAB-00897) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 77 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HSP90 alpha) polyclonal Antibody, Unconjugated (TMAB-00897) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Sample: Testis (Mouse) Lysate at 40 μg
Primary: Anti-HSP90 alpha (TMAB-00897) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 98 kDa
4. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Human U251 cell lysates
Lane 4: Human Molt-4 cell lysates
Primary: Anti-HSP90 alpha (TMAB-00897) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 90 kDa
5. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP90 alpha) Polyclonal Antibody, Unconjugated (TMAB-00897) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ICC/IF=1:100-500; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human HSP90 alpha

Human

HSP90AA1

Heat shock protein HSP 90-alpha

MMPs,Tumor biomarkers,Heat Shock Proteins,Organelle Proteins

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.