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Anti-HSP90AA1 Polyclonal Antibody
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产品编号 TMAB-00897
Anti-HSP90AA1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HSP90AA1。Anti-HSP90AA1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-HSP90AA1 Polyclonal Antibody
Anti-HSP90AA1 Polyclonal Antibody
还可以产品编号 TMAB-00897
Anti-HSP90AA1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HSP90AA1。Anti-HSP90AA1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-HSP90AA1 Polyclonal Antibody is a Rabbit antibody targeting HSP90AA1. Anti-HSP90AA1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 验证活性 | 1. Sample: 293t (Human) Cell Lysate at 30 μg 293T KO HSP90 alpha (Human) Cell Lysate at 30 μg Primary: Anti-HSP90 alpha (TMAB-00897) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 77 kDa 2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HSP90 alpha) polyclonal Antibody, Unconjugated (TMAB-00897) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-HSP90 alpha (TMAB-00897) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 98 kDa 4. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human Molt-4 cell lysates Primary: Anti-HSP90 alpha (TMAB-00897) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 90 kDa 5. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP90 alpha) Polyclonal Antibody, Unconjugated (TMAB-00897) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Hsp90 (heat shock protein 90) is a molecular chaperone and is one of the most abundant proteins in unstressed cells. It is an ubiquitous molecular chaperone found in eubacteria and all branches of eukarya, but it is apparently absent in archaea. Whereas cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions. |
| 免疫原 | KLH conjugated synthetic peptide: human HSP90 alpha |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | MMPs,Tumor biomarkers,Heat Shock Proteins,Organelle Proteins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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