Anti-HSP60 Polyclonal Antibody is a Rabbit antibody targeting HSP60. Anti-HSP60 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (TMAB-00892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (TMAB-00892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (TMAB-00892) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Adrenal gland (Mouse) Lysate at 40 μg
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 4: Adrenal gland (Rat) Lysate at 40 μg
Lane 5: Jurkat (Human) Cell Lysate at 30 μg
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 7: HepG2 (Human) Cell Lysate at 30 μg
Lane 8: Hela (Human) Cell Lysate at 30 μg
Lane 9: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-HSP60 (TMAB-00892) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
5. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (TMAB-00892) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HSP60

Human

Signal transduction proteins ELISA kits,Heat Shock Proteins,Mitochondria