Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.

Monoclonal Mouse IgG1

8B935

Human

1. Immunofluorescence staining of Human HSP27 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human HSP27 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody and countstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human HSP27 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

FCM,ICC/IF

ICC-IF: 1:20-1:100; FCM: 1:25-1:100

Monoclonal

Unconjugated

FITC

2

Recombinant Human HSP27 / HSPB1 protein (TMPY-00823)

Human

Cancer Drug Targets