Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.

Monoclonal Rabbit IgG

7V28

Human

1. HSP27 was immunoprecipitated using:

-Lane A:0.5 mg Caco-2 Whole Cell Lysate.

-2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-HSP27 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 25 kDa.

-Observed band size: 25 kDa.

2. Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:23 kDa.

-Observed band size:26 kDa

WB,ELISA,ELISA(Cap),IP

WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000

Monoclonal

Unconjugated

Recombinant Human HSP27 protein (TMPY-00823)

Human

Cancer Drug Targets