Anti-Hsp27/HSPB1 Antibody (2O259) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (2O259) can be used in FCM,ICC/IF.

Monoclonal Rabbit IgG

2O259

Human

1. Immunofluorescence staining of Human HSP27 in A431 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HSP27 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human HSP27 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

FCM,ICC/IF

ICC-IF: 1:20-1:100; FCM: 1:25-1:100

Monoclonal

Unconjugated

FITC/PE

3

Recombinant Human HSP27 protein (TMPY-00823)

Human

Cancer Drug Targets