Anti-HRH1 Polyclonal Antibody is a Rabbit antibody targeting HRH1. Anti-HRH1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Human (predicted:Mouse,Rat,Dog,Pig,Cow)

1. Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 μg
Lane 2: U251 (Human) Cell Lysate at 30 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
2. Sample:
LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (TMAB-00889) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HRH1

Human

HRH1

Histamine H1 receptor

Allergens,Macrophage / Inflamm.,Hormones,GPCR

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.