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Anti-HRH1 Polyclonal Antibody
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产品编号 TMAB-00889
Anti-HRH1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HRH1。Anti-HRH1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-HRH1 Polyclonal Antibody
Anti-HRH1 Polyclonal Antibody
还可以产品编号 TMAB-00889
Anti-HRH1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HRH1。Anti-HRH1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-HRH1 Polyclonal Antibody is a Rabbit antibody targeting HRH1. Anti-HRH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| 交叉反应 | Human (predicted:Mouse,Rat,Dog,Pig,Cow) |
| 验证活性 | 1. Sample: Lane 1: U87MG (Human) Cell Lysate at 30 μg Lane 2: U251 (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Lane 4: Hela (Human) Cell Lysate at 30 μg Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 2. Sample: LOVO (Human) Cell Lysate at 30 μg Primary: Anti-HRH1 (TMAB-00889) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (TMAB-00889) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]. |
| 免疫原 | KLH conjugated synthetic peptide: human HRH1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Allergens,Macrophage / Inflamm.,Hormones,GPCR |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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