Anti-HNF4A Polyclonal Antibody is a Rabbit antibody targeting HNF4A. Anti-HNF4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
BRL-3a (Rat) Cell Lysate at 30 μg
Primary: Anti-HNF4A (TMAB-00877) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HNF4A) Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample: Hcclm3 Cell (Human) Lysate at 40 μg
Primary: Anti-HNF4A (TMAB-00877) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HNF4 alpha

Human

Energy Metabolism,Heart disease,Diabetes,Cancer,Cytochromes,Lipid metabolism,Lipases,Cholesterol Metabolism,Energy Metabolism,Pol II Transcription,Liver development,Cytochromes,Cholesterol Metabolism,Extrinsic,Diabetes associated,Metabolism of lipids and lipoproteins