Anti-HNF4A Antibody (3W706) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (3W706) can be used in WB,ICC/IF.

IgG

3W706

Human

1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HNF4A) monoclonal Antibody, unconjugated (TMAB-07205) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with HNF4A monoclonal antibody, unconjugated (TMAB-07205) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human HNF 4 alpha

Human

HNF4A

Hepatocyte nuclear factor 4-alpha

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.