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Anti-HMGA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 HMGA1。Anti-HMGA1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
Anti-HMGA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 HMGA1。Anti-HMGA1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-HMGA1 Polyclonal Antibody is a Rabbit antibody targeting HMGA1. Anti-HMGA1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human (predicted:Mouse,Rat,Chicken,Cow) |
验证活性 | 1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus. Chromosome. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex. |
免疫原 | KLH conjugated synthetic peptide: human HMGA1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Tumor biomarkers,DNA / RNA binding,Base Excision Repair,Transcription Factors,Host Virus Interaction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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