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Anti-HMGA1 Polyclonal Antibody

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Anti-HMGA1 Polyclonal Antibody
产品编号 TMAB-00869

Anti-HMGA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 HMGA1。Anti-HMGA1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

Anti-HMGA1 Polyclonal Antibody

Anti-HMGA1 Polyclonal Antibody

Rating icon 还可以
Anti-HMGA1 Polyclonal Antibody
产品编号 TMAB-00869

Anti-HMGA1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 HMGA1。Anti-HMGA1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-HMGA1 Polyclonal Antibody is a Rabbit antibody targeting HMGA1. Anti-HMGA1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human (predicted:Mouse,Rat,Chicken,Cow)
验证活性
1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
应用
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus. Chromosome.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.
抗原信息
免疫原
KLH conjugated synthetic peptide: human HMGA1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Tumor biomarkers,DNA / RNA binding,Base Excision Repair,Transcription Factors,Host Virus Interaction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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