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Anti-Histone H3 Antibody (2C936)
还可以产品编号 TMAY-00019
Anti-Histone H3 Antibody (2C936) 是一种 Mouse 抗体，靶向 Histone H3。Anti-Histone H3 Antibody (2C936) 可用于 ICC/IF。
Anti-Histone H3 Antibody (2C936)
Anti-Histone H3 Antibody (2C936)
还可以产品编号 TMAY-00019
Anti-Histone H3 Antibody (2C936) 是一种 Mouse 抗体，靶向 Histone H3。Anti-Histone H3 Antibody (2C936) 可用于 ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Histone H3 Antibody (2C936) is a Mouse antibody targeting Histone H3. Anti-Histone H3 Antibody (2C936) can be used in ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 2C936 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of histone H3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human histone H3 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nucleus. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the C-terminus of the Human Histone H3 |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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