Anti-His Tag Antibody (6Y42) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (6Y42) can be used in IF,IP,WB.

IgG1

6Y42

1. IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
2. 2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000.
3. IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with His-tag fusion protein 3) IP (untransfected 293+anti-His mAb+ Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG.

WB: 1:3000; IP: 1:200; IF: 1:1000

Monoclonal

Unconjugated

KLH conjugated synthetic peptide: HHHHHH