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Anti-His Tag Antibody (3U153) 是一种 Mouse 抗体,靶向 His Tag。Anti-His Tag Antibody (3U153) 可用于 ELISA。
Anti-His Tag Antibody (3U153) 是一种 Mouse 抗体,靶向 His Tag。Anti-His Tag Antibody (3U153) 可用于 ELISA。
产品描述 | Anti-His Tag Antibody (3U153) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (3U153) can be used in ELISA. |
Ig Type | Monoclonal Mouse IgG2b |
克隆号 | 3U153 |
交叉反应 | other |
应用 | ELISA |
推荐剂量 | ELISA: 1:5000-1:20000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The hexahistidine tag (6× His-tag) is the most frequently used affinity tag for protein enrichment. His-tagged proteins can be purified easily by the chelated metal ions as affinity ligands. The basis for affinity purification is known as immobilized metal affinity chromatography (IMAC). His-tag can bind best to IMAC resin in near-neutral buffer conditions (physiologic pH and ionic strength), and thus the fusion proteins can be eluted with binding buffer containing certain concentrations of imidazole. If possible, the elution is also accomplished with low pH (e.g., 0.1 M glycine-HCl, pH 2.5) or an excess of strong chelator (e.g., EDTA). |
偶联 | Unconjugated |
其他可选修饰符 | HRP |
可提供抗体种类 | 2 |
免疫原 | A synthetic peptide corresponding to 6×His |
抗原种属 | other |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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