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Anti-His Polyclonal Antibody 是一种 Rabbit 抗体,靶向 His。Anti-His Polyclonal Antibody 可用于 WB。
Anti-His Polyclonal Antibody 是一种 Rabbit 抗体,靶向 His。Anti-His Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,330 | 5日内发货 | |
100 μL | ¥ 2,195 | 5日内发货 |
产品描述 | Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB. |
别名 | His Tag, HHHHHH, 6histag, 6*His |
Ig Type | IgG |
验证活性 | 1. Gel: 8%SDS-PAGE, Lysate: 2ug Recombinant 6-HIS Tagged protein, Primary antibody: 1/500 dilution, Secondary antibody dilution: 1/8000, Exposure time: 5 seconds. |
应用 | |
推荐剂量 | WB: 1:500-2000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antigen affinity purification. |
性状 | Liquid |
缓冲液 | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
研究背景 | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
偶联 | Unconjugated |
免疫原 | KLH conjugated synthetic peptide: 18XHis |
分子量 | Actual: 46 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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