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别名 His Tag, HHHHHH, 6histag, 6*His
Anti-His Polyclonal Antibody 是一种 Rabbit 抗体,靶向 His。Anti-His Polyclonal Antibody 可用于 WB。
Anti-His Polyclonal Antibody 是一种 Rabbit 抗体,靶向 His。Anti-His Polyclonal Antibody 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,330 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
| 产品描述 | Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB. |
| 别名 | His Tag, HHHHHH, 6histag, 6*His |
| Ig Type | IgG |
| 验证活性 | 1. Gel: 8%SDS-PAGE, Lysate: 2ug Recombinant 6-HIS Tagged protein, Primary antibody: 1/500 dilution, Secondary antibody dilution: 1/8000, Exposure time: 5 seconds. ![]() |
| 应用 | WB |
| 推荐剂量 | WB: 1:500-2000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Antigen affinity purification. |
| 性状 | Liquid |
| 缓冲液 | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
| 研究背景 | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
| 偶联 | Unconjugated |
| 免疫原 | KLH conjugated synthetic peptide: 18XHis |
| 分子量 | Actual: 46 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |