Anti-HIPK2 Antibody (1O971) is a Rabbit antibody targeting HIPK2. Anti-HIPK2 Antibody (1O971) can be used in WB,ICC/IF,IP,FCM.

IgG

1O971

Human (predicted:Mouse)

1. Western blot analysis of HT-1080 cell lysate. Using HIPK2 (TMAB-07047) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HIPK2) monoclonal Antibody, unconjugated (TMAB-07047) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HIPK2 antibody (TMAB-07047, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human HIPK2

Human

Hipk2

Homeodomain-interacting protein kinase 2