Anti-HIF-3 alpha Polyclonal Antibody is a Rabbit antibody targeting HIF-3 alpha. Anti-HIF-3 alpha Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

IgG

Human,Mouse,Rat (predicted:Horse,Cow,Pig,Dog)

1. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated (TMAB-00860) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HIF3 alpha) polyclonal Antibody, Unconjugated (TMAB-00860) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human HIF3 alpha

Human

HIF,HIF,HIF,HLH