Anti-HGFR/c-Met Antibody (6P935) is an antibody targeting HGFR/c-Met. Anti-HGFR/c-Met Antibody (6P935) can be used in ELISA, IF, FC.

Mouse IgG2a

6P935

Human

1. Immunofluorescence staining of SH-SY5Y cell with TMAH-00749 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
2. Overlay Peak curve showing Hela cells surface stained with TMAH-00749 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IF, FC

IF:1:20-1:200; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human MET Protein

Human

4233

Epigenetics and Nuclear Signaling, Cancer, Signal transduction