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Anti-HGF Polyclonal Antibody
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产品编号 TMAB-00852
Anti-HGF Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HGF。Anti-HGF Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-HGF Polyclonal Antibody
Anti-HGF Polyclonal Antibody
还可以产品编号 TMAB-00852
Anti-HGF Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HGF。Anti-HGF Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-HGF Polyclonal Antibody is a Rabbit antibody targeting HGF. Anti-HGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (HGF) Polyclonal Antibody, Unconjugated (TMAB-00852) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Lane 1: Rat Placenta tissue lysates Lane 2: Rat Cerebellum tissue lysates Primary: Anti-HGF (TMAB-00852) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/80 kDa Observed band size: 60 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:200-500; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | HGF is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. HGF regulates many physiological processes including proliferation, scattering, morphogenesis and survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. This recombinant human HGF is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of the monkey epithelial cell line 4MBr-5. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 免疫原 | KLH conjugated synthetic peptide: human HGF alpha |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Hormones,Angiogenic growth factors |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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